|
KMID : 0545120100200010187
|
|
Journal of Microbiology and Biotechnology
2010 Volume.20 No. 1 p.187 ~ p.192
|
|
|
Investigation of possible horizontal gene transfer from transgenic rice to soil microorganisms in paddy rice field
|
|
Kim Sung-Eun
Moon Jae-Sun
Kim Jung-Kyu
Choi Won-Sik
Kim Sung-Uk
Lee Sang-Han
|
|
|
Abstract
|
|
|
|
In order to monitor the possibility of horizontal gene transfer between transgenic rice and microorganisms in paddy rice field, the gene flow from bifunctional fusion (TPSP) rice containing trehalose-6-phosphate synthase and phosphatase to microorganisms in soils was investigated. The soil samples collected every month from the paddy rice field during June, 2004 to March, 2006 were investigated by multiplex PCR, Southern hybridization, and amplified fragment length polymorphism (AFLP). The TPSP gene from soil genomics DNAs was not detected by PCR. Soil genomic DNAs were not shown its homologies on the Southern blotting data, indicating that gene-transfer did not occur during the last two years in paddy rice field. In addition, the AFLP band patterns produced by both soil genomic DNAs extracted from transgenic and non-transgenic rice field appeared similar to each other when analyzed by NTSYSpc program. Thus, these data suggest that transgenic rice does not give a significant impact on the communities of soil microorganisms although long-term observation may be needed.
|
|
|
KEYWORD
|
|
|
Horizontal gene transfer, transgenic TPSP rice, soil microorganisms, AFLP, environmental risk assessment
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|